Optical imaging in thick samples

نویسندگان

چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Deep and Clear Optical Imaging of Thick Inhomogeneous Samples

Inhomogeneity in thick biological specimens results in poor imaging by light microscopy, which deteriorates as the focal plane moves deeper into the specimen. Here, we have combined selective plane illumination microscopy (SPIM) with wavefront sensor adaptive optics (wao). Our waoSPIM is based on a direct wavefront measure using a Hartmann-Shack wavefront sensor and fluorescent beads as point s...

متن کامل

Multilayer three-dimensional super resolution imaging of thick biological samples.

Recent advances in optical microscopy have enabled biological imaging beyond the diffraction limit at nanometer resolution. A general feature of most of the techniques based on photoactivated localization microscopy (PALM) or stochastic optical reconstruction microscopy (STORM) has been the use of thin biological samples in combination with total internal reflection, thus limiting the imaging d...

متن کامل

Three-Dimensional, Tomographic Super-Resolution Fluorescence Imaging of Serially Sectioned Thick Samples

Three-dimensional fluorescence imaging of thick tissue samples with near-molecular resolution remains a fundamental challenge in the life sciences. To tackle this, we developed tomoSTORM, an approach combining single-molecule localization-based super-resolution microscopy with array tomography of structurally intact brain tissue. Consecutive sections organized in a ribbon were serially imaged w...

متن کامل

Blind Structured Illumination Microscopy Reconstruction in Thick Samples

Aurélie Jost, Elen Tolstik, Bo-Jui Chang, Ernst H.K. Stelzer, Anne Sentenac and Rainer Heintzmann Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-SchillerUniversity Jena, Helmholtzweg 4, 07743 Jena, Germany Leibniz Institute of Photonic Technology, Albert-Einstein Str. 9, 07745 Jena, Germany BMLS, Physical Biology, Goethe University, D-60438 Frankfurt am Main, Germany Insti...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Nature Methods

سال: 2008

ISSN: 1548-7091,1548-7105

DOI: 10.1038/nmeth.f.241